KSR2 Kinase Assay Service

Activity-based KSR2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
KSR2
Enzyme
Human KSR2
HGNC
KSR2
Synonym
Kinase suppressor of Ras 2
Substrates
Peptide substrate, [KRREILSRRPSYR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine6,400
Ro 31-822017,100
GW507411,300
H-89>100,000
Rottlerin>100,000
Scientific Information
Q6VAB6 (http://www.uniprot.org/uniprot/Q6VAB6)
Screening Location
Malvern, USA
Reference compound IC50 for KSR2
Reference compound IC50 for KSR2