Activity-based LATS1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LATS1
Enzyme
Human LATS1
HGNC
LATS1
Synonym
WARTS, wts
Substrates
Peptide substrate, [KKRNRRLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.5 |
| Ro 31-8220 | 10 |
| GW 5074 | 14,000 |
| H-89 | 500 |
| Rottlerin | >50,000 |
Scientific Information
O95835 (http://www.uniprot.org/uniprot/Q95835)
Screening Location
Malvern, USA
Reference compound IC50 for LATS1
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