Activity-based LATS2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LATS2
Enzyme
Human LATS2
HGNC
LATS2
Synonym
FLJ13161, LATS
Substrates
Peptide substrate, [KKRNRRLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.6 |
| Ro 31-8220 | 15 |
| GW 5074 | 1,700 |
| H-89 | 600 |
| Rottlerin | >50,000 |
Scientific Information
Q9NRM7 (http://www.uniprot.org/uniprot/Q9NRM7)
Screening Location
Malvern, USA
Reference compound IC50 for LATS2
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