LIMK1 Kinase Assay Service

Activity-based LIMK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
LIMK1

Enzyme
Human LIMK1

HGNC
LIMK1

Synonym
LIMK

Substrates
Cofilin 1, 3 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.7
Ro 31-8220 7,100
GW5074 12,000
H-89 1,300
Rottlerin >50,000
Scientific Information
P53667 (http://www.uniprot.org/uniprot/P53667)

Screening Location
Malvern, USA

Reference compound IC50 for LIMK1
Reference compound IC50 for LIMK1

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