Activity-based LIMK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
LIMK2
Enzyme
Human LIMK2, wt
HGNC
LIMK2
Manning ID
LIMK2
Synonym
LIMK2
UniProt
P53671 (LIMK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
CFL1, ATP
Reaction
CFL1 + 33P-ATP = 33P-CFL1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| NH125 | 4800 |
| Sorafenib | 7300 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | LIMK2 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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