LOK/STK10 Kinase Assay Service

Activity-based LOK/STK10 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
LOK/STK10

Enzyme
Human LOK/STK10

HGNC
STK10

Synonym
LOK, PRO2729

Substrates
Peptide substrate, [RLGRDKYKTLRQIRQ], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.5
Ro 31-8220 45
GW5074 1,900
H-89 9,300
Rottlerin >100,000
Scientific Information
O94804 (http://www.uniprot.org/uniprot/O94804)

Screening Location
Malvern, USA

Reference compound IC50 for LOK/STK10
Reference compound IC50 for LOK/STK10