LOK/STK10 Kinase Assay Service

Activity-based LOK/STK10 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LOK/STK10
Enzyme
Human LOK/STK10
HGNC
STK10
Synonym
LOK, PRO2729
Substrates
Peptide substrate, [RLGRDKYKTLRQIRQ], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine<0.5
Ro 31-822045
GW50741,900
H-899,300
Rottlerin>100,000
Scientific Information
O94804 (http://www.uniprot.org/uniprot/O94804)
Screening Location
Malvern, USA
Reference compound IC50 for LOK/STK10
Reference compound IC50 for LOK/STK10