LRRK2 (I2020T) Kinase Assay Service

Activity-based LRRK2 (I2020T) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LRRK2 (I2020T)
Enzyme
Human LRRK2 (I2020T)
HGNC
LRRK2 (I2020T)
Synonym
PARK8, ROCO2, FLJ45829, DKFZp434H2111
Substrates
Peptide substrate, [RLGRDKYKTLRQIRQ], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine3.0
Ro 31-8220380
GW507439
H-89>100,000
Rottlerin>100,000
Scientific Information
Q5S007 (http://www.uniprot.org/uniprot/Q5S007)
Screening Location
Malvern, USA
Reference compound IC50 for LRRK2 (I2020T)
Reference compound IC50 for LRRK2 (I2020T)