M1 muscarinic Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
M1 Binding
GPCR Target
Human M1
Source
Membrane preparation from HEK293 cells stably transfected with human M1 receptor.
Scientific Information
ACM1 (https://www.uniprot.org/uniprotkb/P11229/entry)
Assay Properties
Protocol
Radioligand filter binding to M1 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Radioligand
Pirenzepine, 5 nM
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| Pirenzepine | 9.5 |
| AF-DX 384 | >10,000 |
| Atropine | 0.96 |
| 4-DAMP | 0.85 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound binding data for M1
PDF