M5 muscarinic Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
M5 Binding
GPCR Target
Human M5
Source
Membrane preparation from CHO-K1 cells stably transfected with human M5 receptor.
Scientific Information
ACM5 (https://www.uniprot.org/uniprotkb/P08912/entry)
Assay Properties
Protocol
Radioligand filter binding to M5 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter.
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Radioligand
NMS, 10 nM
Reference Compound IC50
| Compound | IC50 (nM) |
|---|---|
| Atropine | 3.8 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website GPCR Assay and Profiling Services
Reference compound binding data for M5
PDF