MAP2K6 NanoBRET Kinase Assay

MAP2K6 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target

MAP2K6

HGNC Symbol

MAP2K6

Synonyms

MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-MAP2K6 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-11 and then treated with reference compound APY-69 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
APY-69 5.779e-008
Screening Location

Malvern, USA

Reference compound IC50 for MAP2K6