Activity-based MAP4K3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MAP4K3
Enzyme
Human MAP4K3, wt
HGNC
MAP4K3
Manning ID
KHS2
Synonym
GLK, MAPKKKK3, RAB8IPL1
UniProt
Q8IVH8 (M4K3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
CDCA8, ATP
Reaction
CDCA8 + 33P-ATP = 33P-CDCA8 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.1 |
| Sorafenib | >10000 |
| Sunitinib | 86 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MAP4K3 |
| wt Profiler Panel | Yes |
| IC50 wt Profiler Panel | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MAP4K3 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 2051-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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