MAP4K4 Kinase Activity Assay Service

Activity-based MAP4K4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

MAP4K4

Enzyme

Human MAP4K4, wt

HGNC

MAP4K4

Manning ID

ZC1/HGK

Synonym

FLH21957, FLJ20373, HGK, KIAA0687, NIK, MEKKK4, MAP4K4, HGK/MAP4K4

UniProt

O95819 (M4K4_HUMAN)

Kinase Type

Protein kinase

Kinase Group

STE

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Casein, ATP

Reaction

Casein + 33P-ATP = 33P-Casein + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 13
Sorafenib 10000
Sunitinb 2500

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice MAP4K4
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – MAP4K4
(Activity verified by 33PanQinaseTM-Assay)

Product Number 1493-0000-1
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