MAPKAPK2 Kinase Assay Service

Activity-based MAPKAPK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MAPKAPK2

Enzyme
Human MAPKAPK2

HGNC
MAPKAPK2

Synonym
MAPKAPK2

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 196
Ro 31-8220 22,000
GW5074 >25,000
H-89 >25,000
Scientific Information
P49137 (http://www.uniprot.org/uniprot/P49137)

Screening Location
Malvern, USA

Reference compound IC50 for MAPKAPK2
Reference compound IC50 for MAPKAPK2

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