MAPKAPK3 Kinase Assay Service

Activity-based MAPKAPK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MAPKAPK3
Enzyme
Human MAPKAPK3
HGNC
MAPKAPK3
Synonym
3PK, MAPKAP, MAPKAP3
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine4,000
GW 50746,400
Scientific Information
Q16644 (http://www.uniprot.org/uniprot/Q16644)
Screening Location
Malvern, USA
Reference compound IC50 for MAPKAPK3
Reference compound IC50 for MAPKAPK3