MAPKAPK5/PRAK Kinase Assay Service

Activity-based MAPKAPK5/PRAK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MAPKAPK5/PRAK
Enzyme
Human MAPKAPK5/PRAK
HGNC
MAPKAPK5
Synonym
PRAK
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine420
Ro 31-822015,000
GW 507413,000
H-89>20,000
Rottlerin>20,000
Scientific Information
Q8IW41 (http://www.uniprot.org/uniprot/Q8IW41)
Screening Location
Malvern, USA
Reference compound IC50 for MAPKAPK5/PRAK
Reference compound IC50 for MAPKAPK5/PRAK