MARK1 Kinase Assay Service

Activity-based MARK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MARK1

Enzyme
Human MARK1

HGNC
MARK1

Synonym
KIAA1477

Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.5
Ro 31-8220 6,100
GW 5074 3,500
H-89 5,400
Scientific Information
Q9P0L2 (http://www.uniprot.org/uniprot/Q9P0L2)

Screening Location
Malvern, USA

Reference compound IC50 for MARK1
Reference compound IC50 for MARK1

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