MARK2/PAR-1Ba Kinase Assay Service

Activity-based MARK2/PAR-1Ba kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MARK2/PAR-1Ba
Enzyme
Human MARK2/PAR-1Ba
HGNC
MARK2
Synonym
EMK1, MGC99619, PAR-1Ba, ELKL
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine<0.5
Ro 31-82201,800
GW 50743,300
H-892,300
Scientific Information
Q7KZI7-4 (http://www.uniprot.org/uniprot/Q7KZI7-4)
Screening Location
Malvern, USA
Reference compound IC50 for MARK2/PAR-1Ba
Reference compound IC50 for MARK2/PAR-1Ba
PDF