Activity-based MARK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MARK3
Enzyme
Human MARK3
HGNC
MARK3
Synonym
CTAK1, EMK2, Protein kinase STK10, Par-1a, Serine/threonine-protein kinase p78
Scientific Information
P27448 (http://www.uniprot.org/uniprot/P27448)
Assay Properties
Substrates
Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 0.02 nM |
| Ro 31-8220 | 1.56 µM |
| GW 5074 | 2.67 µM |
| H-89 | 5.27 µM |
| Sunitinib | 141 nM |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of MARK3
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