Activity-based MASTL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MASTL
Enzyme
Human MASTL
HGNC
MASTL
Synonym
GLW, GREATWALL, GWL, hGWL, THC2
Substrates
ARPP19, 5 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 39 |
| Ro 31-8220 | 9,400 |
| GW 5074 | >100,000 |
| H-89 | 5,800 |
| Rottlerin | >100,000 |
Scientific Information
Q96GX5 (http://www.uniprot.org/uniprot/Q96GX5)
Screening Location
Malvern, USA
Reference compound IC50 for MASTL
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