MASTL Kinase Assay Service

Activity-based MASTL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MASTL
Enzyme
Human MASTL
HGNC
MASTL
Synonym
GLW, GREATWALL, GWL, hGWL, THC2
Substrates
ARPP19, 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine39
Ro 31-82209,400
GW 5074>100,000
H-895,800
Rottlerin>100,000
Scientific Information
Q96GX5 (http://www.uniprot.org/uniprot/Q96GX5)
Screening Location
Malvern, USA
Reference compound IC50 for MASTL
Reference compound IC50 for MASTL