Activity-based MASTL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MASTL
Enzyme
Human MASTL
HGNC
MASTL
Synonym
Microtubule-associated serine/threonine-protein kinase-like, GW, GWL, THC2
Scientific Information
Q96GX5 (http://www.uniprot.org/uniprot/Q96GX5)
Assay Properties
Substrates
Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 85.6 nM |
| Ro 31-8220 | 18.7 µM |
| GW 5074 | 13.6 µM |
| H-89 | 15.6 µM |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of MASTL
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