MCL1 Apoptosis Assay for compound screening and profiling using HTRF to measure compound binding to the target.
Target Overview
CAT#
MCL1
Enzyme
Human MCL1
Synonyms
Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, BCL2L3
Substrates
BAK peptide, 100 nM; or Noxa peptide, 50 nM
Assay Properties
Reaction
Competition binding assay with Bak or Noxa peptide in HTRF assay.
Reference Compound IC50s
Bak Peptide
| Compounds | IC50 (nM) |
|---|---|
| ABT-263 | 134 |
| ABT-737 | 860 |
| ABT-199 | 390 |
| A-1210477 | 5.9 |
Noxa Peptide
| Compounds | IC50 (nM) |
|---|---|
| ABT-263 | ND |
| ABT-737 | ND |
| ABT-199 | ND |
| A-1210477 | 6.6 |
Scientific Information
Q07820 (http://www.uniprot.org/uniprot/Q07820)
Screening Location
Malvern, USA
BAK peptide displacement IC50 for MCL1
Noxa peptide displacement IC50 for MCL1
