MCL1 Apoptosis Assay Service

MCL1 Apoptosis Assay for compound screening and profiling using HTRF to measure compound binding to the target.

Target Overview
CAT#

MCL1

Enzyme

Human MCL1

Synonyms

Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, BCL2L3

Substrates

BAK peptide, 100 nM; or Noxa peptide, 50 nM

Assay Properties
Reaction

Competition binding assay with Bak or Noxa peptide in HTRF assay.

Reference Compound IC50s

Bak Peptide

Compounds IC50 (nM)
ABT-263 134
ABT-737 860
ABT-199 390
A-1210477 5.9

Noxa Peptide

Compounds IC50 (nM)
ABT-263 ND
ABT-737 ND
ABT-199 ND
A-1210477 6.6
Scientific Information

Q07820 (http://www.uniprot.org/uniprot/Q07820)

Screening Location

Malvern, USA

BAK peptide displacement IC50 for MCL1

BAK peptide displacement IC50 for MCL1

Noxa peptide displacement IC50 for MCL1

Noxa peptide displacement IC50 for MCL1