mDGKA Kinase Assay Service

Activity-based mDGKA lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.

Target Overview
CAT#

mDGKA

Enzyme

Mouse DGKA

HGNC

mDGKA

Synonym

Diacylglycerol kinase alpha, DAG kinase alpha, 80 kDa diacylglycerol kinase, DAGK1

Scientific Information

O88673 (https://www.uniprot.org/uniprot/O88673)

Assay Properties
Substrates

DLG (1, 2-Dilauroyl-sn-glycerol), 500 µM + CaCl2, 2 mM
ATP, 50 µM

Reaction

1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+

Detection

ADP-Glo Assay

Reference Compound IC50s
Compounds IC50 (nM)
DGK-IN-1 (CAS No: 2407892-34-4) 19.9
Calphostin C 1,042
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for mDGKA

Reference compound IC50 for mDGKA