MEK1 (P124L) Kinase Assay Service

Activity-based MEK1 (P124L) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MEK1 (P124L)

Enzyme
Human MEK1 (P124L)

HGNC
MAP2K1 (P124L)

Synonym
MAPKK1, MKK1, PRKMK1

Substrates
ERK2 (K52R), 3 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.1
Ro 31-8220 19,000
GW 5074 3,100
H-89 25,000
Rottlerin 12,000
Scientific Information
Q02750 (http://www.uniprot.org/uniprot/Q02750)

Screening Location
Malvern, USA

Reference compound IC50 for MEK1 (P124L)
Reference compound IC50 for MEK1 (P124L)

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