MEK5 Kinase Assay Service

Activity-based MEK5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MEK5

Enzyme
Human MEK5

HGNC
MAP2K5

Synonym
MAPKK5, MKK5, PRKMK5

Substrates
ERK5 (K84R), 3 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 52
Ro 31-8220 1,100
GW5074 15,000
H-89 >100,000
Rottlerin >100,000
Scientific Information
Q13163 (http://www.uniprot.org/uniprot/Q13163)

Screening Location
Malvern, USA

Reference compound IC50 for MEK5
Reference compound IC50 for MEK5