Activity-based MEKK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEKK2
Enzyme
Human MEKK2
HGNC
MAP3K2
Synonym
MAPKKK2, MAP3K2
Scientific Information
Q9Y2U5 (http://www.uniprot.org/uniprot/Q9Y2U5)
Assay Properties
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 6.3 nM |
| Ro 31-8220 | 161 nM |
| GW 5074 | 5.3 µM |
| H-89 | >100 µM |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of MEKK2
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