Activity-based MEKK6 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEKK6
Enzyme
Human MEKK6
HGNC
MAP3K6
Synonym
M3K6, ASK2, MAPKKK6, MEKK6
Scientific Information
O95382 (http://www.uniprot.org/uniprot/O95382)
Assay Properties
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 |
|---|---|
| Staurosporine | 556 nM |
| Ro 31-8220 | >100 µM |
| GW 5074 | 7.4 µM |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Screening Assay Services.
Reference compound inhibition of MEKK6
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