MELK Kinase Assay Service

Activity-based MELK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MELK

Enzyme
Human MELK

HGNC
MELK

Synonym
KIAA0175, PK38

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.7
Ro 31-8220 260
GW 5074 1,200
H-89 1,100
Rottlerin 12,000
Scientific Information
Q14680 (http://www.uniprot.org/uniprot/Q14680)

Screening Location
Malvern, USA

Reference compound IC50 for MELK
Reference compound IC50 for MELK

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