MET D1228H Kinase Activity Assay Service

Activity-based MET D1228H kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

MET D1228H

Enzyme

Human MET D1228H, mutant

HGNC

MET (D1228H)

Manning ID

MET

Synonym

MET, HGFR, RCCP2, c-MET, MET D1228H, c-MET (D1228H)

UniProt

P08581 (MET_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP

Reaction

Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 490
Sorafenib 10000
Sunitinb 30000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice MET D1228H
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 MET Profiler
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – MET D1228H
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0995-0000-1

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