MET Y1230D Kinase Activity Assay Service

Activity-based MET Y1230D kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

MET Y1230D

Enzyme

Human MET Y1230D, mutant

HGNC

MET (Y1230D)

Manning ID

MET

Synonym

MET, HGFR, RCCP2, c-MET, MET Y1230D, c-MET (Y1230D)

UniProt

P08581 (MET_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP

Reaction

Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 240
Sorafenib 10000
Sunitinb 19000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice MET Y1230D
345 wt Profiler No
345 IC50 wt Profiler No
Mutant Profiler Addon Yes
Lipid Kinase Profiler Addon No
IC50 Profiler IC50 MET Profiler
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – MET Y1230D
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0981-0000-1

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