METTL21A Methyltransferase Assay Service

Activity-based METTL21A Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

METTL21A

Enzyme

Human METTL21A

Synonym

Protein N-lysine methyltransferase METTL21A, HSPA lysine methyltransferase, HSPA-KMT, Hepatocellular carcinoma-associated antigen 557b, Methyltransferase-like protein 21A, FAM119A, HCA557B

Substrates

GST-HSPA8-[CTD], 5 µM
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + protein L- lysine = S-adenosyl-L-homocysteine + protein [methyl-³H]-L- lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 72,000
Chaetocin >100,000
Sinefungin >100,000
Suramin 6,200

Scientific Information

Q8WXB1 (http://www.uniprot.org/uniprot/Q8WXB1)

Screening Location

Malvern, USA

Reference compound IC50 for METTL21A

Reference compound IC50 for METTL21A