Activity-based MKK3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MKK3
Enzyme
Human MKK3, wt
HGNC
MAP2K3
Manning ID
MAP2K3
Synonym
MAPKK3; MEK3; MKK3; PRKMK3; SAPK kinase 2; SAPKK-2; SAPKK2
UniProt
P46734 (MP2K3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
p38-alpha KA, ATP
Reaction
p38-alpha KA + 33P-ATP = 33P-p38-alpha KA + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 31 |
| Sorafenib | 10000 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MKK3 |
| 350 wt Profiler | Yes |
| 350 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MKK3 |
| Product Number | 1965-0000-1 |
| Web Shop | Reaction Biology Online Shop |