MKK4 Kinase Assay Service

Activity-based MKK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MKK4

Enzyme
Human MKK4

HGNC
MAP2K4

Synonym
JNKK, JNKK1, MAPKK4, MEK4, PRKMK4, SEK, SERK1

Substrates
JNK1 (K55M), 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 3,200
GW 5074 17,600
Scientific Information
P45985 (http://www.uniprot.org/uniprot/P45985)

Screening Location
Malvern, USA

Reference compound IC50 for MKK4
Reference compound IC50 for MKK4

PDF