MKK7 Kinase Assay Service

Activity-based MKK7 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MKK7

Enzyme
Human MKK7

HGNC
MAP2K7

Synonym
MAPKK7, MEK7, PRKMK7

Substrates
JNK (K55M), 3 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 440
Ro 31-8220 25,000
GW 5074 28,000
H-89 >50,000
Rottlerin >50,000
Scientific Information
O14733 (http://www.uniprot.org/uniprot/O14733)

Screening Location
Malvern, USA

Reference compound IC50 for MKK7
Reference compound IC50 for MKK7