MKK7 Kinase Assay Service

Activity-based MKK7 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MKK7
Enzyme
Human MKK7
HGNC
MAP2K7
Synonym
MAPKK7, MEK7, PRKMK7
Substrates
JNK (K55M), 3 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine440
Ro 31-822025,000
GW 507428,000
H-89>50,000
Rottlerin>50,000
Scientific Information
O14733 (http://www.uniprot.org/uniprot/O14733)
Screening Location
Malvern, USA
Reference compound IC50 for MKK7
Reference compound IC50 for MKK7