MLCK2/MYLK2 Kinase Assay Service

Activity-based MLCK2/MYLK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MLCK2

Enzyme
Human MLCK2

HGNC
MYLK2

Synonym
KMLC, skMLCK, MLCK2

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
Ca2+-Calmodulin, 10 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 18.8
Ro 31-8220 660
GW 5074 2,200
Scientific Information
Q9H1R3 (http://www.uniprot.org/uniprot/Q9H1R3)

Screening Location
Malvern, USA

Reference compound IC50 for MLCK2/MYLK2
Reference compound IC50 for MLCK2/MYLK2

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