MLCK/MYLK Kinase Assay Service

Activity-based MLCK/MYLK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MLCK
Enzyme
Human MLCK
HGNC
MYLK
Synonym
smMLCK, FLJ12216, KRP, MLCK, MLCK108, MLCK210
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine25.6
Ro 31-8220110
GW 5074876
H-8941,600
Rottlerin7,000
Scientific Information
Q15746 (http://www.uniprot.org/uniprot/Q15746)
Screening Location
Malvern, USA
Reference compound IC50 for MLCK/MYLK
Reference compound IC50 for MLCK/MYLK