MLCK/MYLK Kinase Assay Service

Activity-based MLCK/MYLK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MLCK

Enzyme
Human MLCK

HGNC
MYLK

Synonym
smMLCK, FLJ12216, KRP, MLCK, MLCK108, MLCK210

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 25.6
Ro 31-8220 110
GW 5074 876
H-89 41,600
Rottlerin 7,000
Scientific Information
Q15746 (http://www.uniprot.org/uniprot/Q15746)

Screening Location
Malvern, USA

Reference compound IC50 for MLCK/MYLK
Reference compound IC50 for MLCK/MYLK

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