MLK1/MAP3K9 Kinase Assay Service

Activity-based MLK1/MAP3K9 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MLK1/MAP3K9

Enzyme
Human MLK1/MAP3K9

HGNC
MAP3K9

Synonym
MLK1, PRKE1, MEKK9

Substrates
Casein, 1 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.8
Ro 31-8220 300
GW 5074 1,100
H-89 12,000
Scientific Information
P80192 (http://www.uniprot.org/uniprot/P80192)

Screening Location
Malvern, USA

Reference compound IC50 for MLK1/MAP3K9
Reference compound IC50 for MLK1/MAP3K9

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