MLK2/MAP3K10 Kinase Assay Service

Activity-based MLK2/MAP3K10 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MLK2
Enzyme
Human MLK2
HGNC
MAP3K10
Synonym
MLK2, MST, MEKK10
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.5
Ro 31-8220260
GW 50743,200
H-8929,000
Rottlerin32,800
Scientific Information
Q02779 (http://www.uniprot.org/uniprot/Q02779)
Screening Location
Malvern, USA
Reference compound IC50 for MLK2/MAP3K10
Reference compound IC50 for MLK2/MAP3K10