Activity-based MLK4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MLK4
Enzyme
Human MLK4, wt
HGNC
MAP3K21
Manning ID
MLK4
Synonym
KIAA1804, M3KL4, MAP3K21, MLK4
UniProt
Q5TCX8 (M3K21_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RB-CTF, ATP
Reaction
RB-CTF + 33P-ATP = 33P-RB-CTF + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 18 |
| Sorafenib | 10000 |
| Sunitinb | 59000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MLK4 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MLK4 |
| Product Number | 1078-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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