Activity-based MLL1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Human MLL1; 5 component complex
Complex of MLL1: Histone-lysine N-methyltransferase MLL, CXXC-type zinc finger protein 7, ALL1, HTRX, KMT2A, TRX1, WDR5: WD repeat-containing protein 5, BMP2-induced 3-kb gene protein, RBBP5: Retinoblastoma-binding protein 5, Retinoblastoma-binding protein RBQ-3, ASH2L: Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, DPY30: Protein dpy-30 homolog, Dpy-30-like protein
Nucleosomes, 0.05 mg/mlSAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Reference Compound IC50s
Reference compound IC50 for MLL1