MMP 12 Protease Assay Service

MMP 12 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP12
Enzyme
MMP-12, catalytic domain from human recombinant (residues 84-255) expressed in E. coli with C-terminal tag
Synonym
HME, Matrix metalloproteinase-12, MMP-12, Macrophage elastase, ME
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (M)
NNGH2.04E-09
Actinonin1.36E-07
GM60013.07E-10
MMP Inhibitor III2.35E-09
MMP-9/MMP-13 Inhibitor I1.41E-09
Scientific Information
P39900 (http://www.uniprot.org/uniprot/P39900)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-12
Reference compound IC50 for MMP-12