MMP 1 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP1
Enzyme
MMP-1, catalytic domain from human recombinant (residues 81-249) expressed in E. coli with C-terminal tag
Synonym
Matrix metalloproteinase-1, Fibroblast Collagenase
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 7.51E-08 |
| Actinonin | 5.19E-08 |
| GM6001 | 5.19E-10 |
| MMP Inhibitor III | 2.10E-10 |
| MMP-9/MMP-13 Inhibitor I | 4.75E-08 |
Scientific Information
P03956 (http://www.uniprot.org/uniprot/P03956)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-1
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