MMP 2 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
MMP2
Enzyme
MMP-2, catalytic domain from human recombinant (residues 81-423) in E. coli with C-terminal tag
Synonym
Matrix metalloproteinase-2, MMP-2, Gelatinase A, TBE-1
Substrates
(5-FAM/QXLTM) FRET peptide
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (M) |
|---|---|
| NNGH | 2.71E09 |
| Actinonin | 7.50E-08 |
| GM6001 | 1.88E-10 |
| MMP Inhibitor III | 4.70E-10 |
| MMP-9/MMP-13 Inhibitor I | 1.79E-09 |
Scientific Information
P08253 (http://www.uniprot.org/uniprot/P08253)
Screening Location
Malvern, USA
Reference compound IC50 for MMP-2
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