MMP 9 Protease Assay Service

MMP 9 Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview

CAT#
MMP9

Enzyme
MMP-9, catalytic domain from human recombinant (residues 88-438) expressed in E. coli with C-terminal tag

Synonym
MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, GELB

Substrates
(5-FAM/QXLTM) FRET peptide

Assay Properties

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (M)
NNGH 1.43E-09
Actinonin 6.35E-08
GM6001 1.02E-10
MMP Inhibitor III 3.63E-10
MMP-9/MMP-13 Inhibitor I 6.79E-10
Scientific Information
P14780 (http://www.uniprot.org/uniprot/P14780)

Screening Location
Malvern, USA

Reference compound IC50 for MMP-9
Reference compound IC50 for MMP-9