MRCKa/CDC42BPA Kinase Assay Service

Activity-based MRCKa/CDC42BPA kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

MRCKa/CDC42BPA

Enzyme

Human MRCKa/CDC42BPA

HGNC

CDC42BPA

Synonym

MRCK-alpha

Substrates

Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	10.3
Ro 31-8220	2,300

Scientific Information

Q5VT25 (http://www.uniprot.org/uniprot/Q5VT25)

Screening Location

Malvern, USA

Reference compound IC50 for MRCKa/CDC42BPA