MRCKa/CDC42BPA Kinase Assay Service

Activity-based MRCKa/CDC42BPA kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MRCKa/CDC42BPA
Enzyme
Human MRCKa/CDC42BPA
HGNC
CDC42BPA
Synonym
MRCK-alpha
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine10.3
Ro 31-82202,300
Scientific Information
Q5VT25 (http://www.uniprot.org/uniprot/Q5VT25)
Screening Location
Malvern, USA
Reference compound IC50 for MRCKa/CDC42BPA
Reference compound IC50 for MRCKa/CDC42BPA