MRCKb/CDC42BPB Kinase Assay Service

Activity-based MRCKb/CDC42BPB kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

MRCKb/CDC42BP

Enzyme

Human MRCKb/CDC42BP

HGNC

CDC42BPB

Synonym

MRCK-beta, MRCKb

Substrates

Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Staurosporine	9.8
Ro 31-8220	1,100

Scientific Information

Q9Y5S2 (http://www.uniprot.org/uniprot/Q9Y5S2)

Screening Location

Malvern, USA

Reference compound IC50 for MRCKb/CDC42BPB