MSK1/RPS6KA5 Kinase Assay Service

Activity-based MSK1/RPS6KA5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MSK1

Enzyme
Human MSK1

HGNC
RPS6KA5

Synonym
MSK1, RLPK

Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.16
Ro 31-8220 1.9
GW 5074 5,200
H-89 125
Rottlerin 4,400
Scientific Information
O75582 (http://www.uniprot.org/uniprot/O75582)

Screening Location
Malvern, USA

Reference compound IC50 for MSK1
Reference compound IC50 for MSK1

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