MSK2/RPS6KA4 Kinase Assay Service

Activity-based MSK2/RPS6KA4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MSK2
Enzyme
Human MSK2
HGNC
RPS6KA4
Synonym
MSK2, RSK-B
Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.9
Ro 31-822014
GW 50744,900
H-89120
Rottlerin4,400
Scientific Information
O75676 (http://www.uniprot.org/uniprot/O75676)
Screening Location
Malvern, USA
Reference compound IC50 for MSK2
Reference compound IC50 for MSK2