Activity-based MSK2/RPS6KA4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MSK2
Enzyme
Human MSK2
HGNC
RPS6KA4
Synonym
MSK2, RSK-B
Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.9 |
| Ro 31-8220 | 14 |
| GW 5074 | 4,900 |
| H-89 | 120 |
| Rottlerin | 4,400 |
Scientific Information
O75676 (http://www.uniprot.org/uniprot/O75676)
Screening Location
Malvern, USA
Reference compound IC50 for MSK2
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