MSSK1/STK23 Kinase Assay Service

Activity-based MSSK1/STK23 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MSSK1

Enzyme
Human MSSK1

HGNC
SRPK3

Synonym
MSSK1, MGC102944, STK23

Substrates
Peptide substrate, [GRSRSRSRSR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1,750
Ro 31-8220 540
GW 5074 5,800
Rottlerin 11,000
Scientific Information
Q9UPE1 (http://www.uniprot.org/uniprot/Q9UPE1)

Screening Location
Malvern, USA

Reference compound IC50 for MSSK1
Reference compound IC50 for MSSK1

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