MYLK3 Kinase Assay Service

Activity-based MYLK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MYLK3
Enzyme
Human MYLK3
HGNC
MYLK3
Synonym
caMLCK, MLCK
Substrates
Peptide substrate, [KKRPQRRYSNVF], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine130
Ro 31-822041,000
GW 50741,300
H-89>100,000
Rottlerin>100,000
Scientific Information
Q32MK0 (http://www.uniprot.org/uniprot/Q32MK0)
Screening Location
Malvern, USA
Reference compound IC50 for MYLK3
Reference compound IC50 for MYLK3