NEK11 Kinase Assay Service

Activity-based NEK11 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
NEK11

Enzyme
Human NEK11

HGNC
NEK11

Synonym
FLJ23495

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 570
Ro 31-8220 17,800
GW5074 2000
Rottlerin 6,600
Scientific Information
Q8NG66 (http://www.uniprot.org/uniprot/Q8NG66)

Screening Location
Malvern, USA

Reference compound IC50 for NEK11
Reference compound IC50 for NEK11

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