NEK1 Kinase Assay Service

Activity-based NEK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
NEK1
Enzyme
Human NEK1
HGNC
NEK1
Synonym
KIAA1901, NY-REN-55,
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine18.7
Ro 31-82207,700
GW 507424,700
H-8947,000
Scientific Information
Q96PY6 (http://www.uniprot.org/uniprot/Q96PY6)
Screening Location
Malvern, USA
Reference compound IC50 for NEK1
Reference compound IC50 for NEK1